this post was submitted on 14 Nov 2024
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Science Memes

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[–] phdepressed@sh.itjust.works 40 points 23 hours ago (6 children)

Top left is a thermal cycler. Basically it heats and cools samples at a given rate. This is primarily used for generic PCR, and certain enzymatic reactions. Top right is the fancier version of this, it is for qPCR, so it can do the heating and cooling and has a laser/detector for the dye or probe that reacts to generating more dna with each PCR cycle so you can quantify approximately how much of the target DNA you had.

Bottom right is a luminex. This uses detection of fluorophore signals to measure multiple analyates, usually different proteins.

Idk what bottom left is.

[–] runner_g@lemmy.blahaj.zone 7 points 20 hours ago (1 children)

Top left is the CFX96/384, which is also a qPCR instrument.

Bottom left is the 3500 Genetic Analyzer, as someone identified. It's used for sanger sequencing I believe. My last lab had one but I was never trained on it.

[–] phdepressed@sh.itjust.works 4 points 18 hours ago (1 children)

I thought it was just their T100, the CFX96s I've used don't have the touchscreen but yeah the bigger "lid" does look like for the CFX.

[–] runner_g@lemmy.blahaj.zone 2 points 5 hours ago

The bases and hot blocks are interchangable with that series. We recently upgraded to the Opus but our previous CFX96 had the T1000 touch base.

[–] Schmoo@slrpnk.net 11 points 22 hours ago (1 children)

Wow, I know some of those words.

[–] Rednax@lemmy.world 18 points 21 hours ago* (last edited 21 hours ago)

Allow me to translate:

Top left is a thermal cycler. Basically it heats and cools samples at a given rate. This is primarily used for magic. Top right is the fancier version of this, it is for qMagic, so it can do the heating and cooling and has a magic detector for magic components that reacts to magic happening so you can quantify approximately how much magic is going on in the beige box.

Bottom right is a luminex. This uses detection of magic signals to measure magic.

Bottom left is the beige box where magic happens.

[–] jaybone@lemmy.world 4 points 20 hours ago

This guy sciences.

Also username checks out.

[–] StrongHorseWeakNeigh@lemmy.world 5 points 22 hours ago

PCR, aka pipette, cry, repeat

[–] Fuck_u_spez_@sh.itjust.works 2 points 20 hours ago

I know nothing about this kind of lab equipment but Google says the bottom left device is a human DNA sequencer, ABI model 3500.

[–] clay_pidgin@sh.itjust.works 3 points 23 hours ago

Thank you, they sound really specialized!